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| Elulookirjeldus (CV) | ||
| 1. | Eesnimi | Neeme |
| 2. | Perekonnanimi | Tõnisson |
| 3. | Töökoht | Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut Biotehnoloogia õppetool |
| 4. | Ametikoht | vanemteadur |
| 5. | Sünniaeg | 17.02.1971 (päev.kuu.aasta) |
| 6. | Haridus | 1989 Tartu 15. Keskkool 1989 – 1995 Tartu Ülikooli Arstiteaduskond 1995 – 2000 Tartu Ülikooli Arstiteaduskonna doktorant |
| 7. | Teenistuskäik | 1995 - 1996 Tartu Ülikooli Naistekliiniku ekstrakorporaalse viljastamise programmi assistent. 1997 - 2004 TÜ Molekulaarse Biotehnoloogia praktikumi praktiliste tööde juhendaja 1999 - 2001 AS Asper Biotech teadur 2001 - 2002 AS Asper Biotech genotüpiseerimisjuht 2001 - 2002 Euroopa Teadusfondi kursuse “APEX on DNA microarrays” juhendaja 2003 - AS Asper Biotech vanemteadur 2004 TÜ Molekulaar- ja Rakubioloogia Instituudi Biotehnoloogia õppetooli erakorraline teadur (Maris Laane töörühm) 2004 - külalisteadur, Deutsches Krebsforschngszentrum (DKFZ), Heidelberg, Saksamaa 2005 - vanemteadur, Biotehnoloogia õppetool, TÜ Molekulaar- ja Rakubioloogia Instituut |
| 8. | Teaduskraad | Ph.D (molekulaardiagnostika erialal) |
| 9. | Teaduskraadi välja andnud asutus, aasta |
Tartu Ülikool, 2003 |
| 10. | Tunnustused | 2004 - Alexander von Humboldti Fondi / Hertie Fondi Roman Herzogi nimeline teadusstipendium |
| 11. | Teadusorganisatsiooniline ja –administratiivne tegevus |
Eesti Inimesegeneetika Ühingu liige 2000 osalesin genotüpiseerimisrühma eksperdina Eesti Geenivaramu projekti väljatöötamiksel |
| 12. | Juhendamisel kaitstud väitekirjad |
Aune Ahman, MSc, 2003, juh. N. Tõnisson, A. Metspalu. Oligonukleotiidse kiibi valideerimine ja võrdlus teiste TP53 geeni uurimisel kasutatavate meetoditega. Tartu Ülikool Hanno Roomere, MSc, 2002, juh. N. Tõnisson, A. Metspalu. Praimerekstensioonil põhineva resekveneerimismeetodi väljatöötamine tuumorsupressorgeeni TP53 näitel. Tartu Ülikool |
| 13. | Teadustöö põhisuunad | a) Geenikiipidel põhineva molekulaardiagnostika (APEX, arrayed primer extension) arendamine ja ja rakendamine erinevates geenianalüüsi valdkondades b) hüpertensiooni päriliku eelsoodumuse uurimisprojekti käivitamine (prof. M.Laane töörühmas), kandidaatgeenide võrdlev genoomika c) kasvajate molekulaardiagnostika - mutatsiooniuuringud (TÜ, Asper Biotech) ja epigeneetilised muutused (DKFZ) |
| 14. | Jooksvad grandid | |
| 15. | Teaduspublikatsioonid |
Kringen P, Bergamaschi A, Undlien Due E, Wang Y, Menard S, Tagliabue E, Nesland JM, Ahman A, Tõnisson N, Børresen-Dale AL. Evaluation of Arrayed Primer Extension (APEX) in TP53 mutation detection in breast and ovarian carcinomas. Biotechniques 2005; 39(5):755-61 Le Calvez F, Ahman A, Tonisson N, Lambert J, Temam S, Brennan P, Zaridze DG, Metspalu A, Hainaut P. Arrayed Primer Extension Resequencing of Mutations in the TP53 Tumor Suppressor Gene: Comparison with Denaturing HPLC and Direct Sequencing. Clin Chem. 2005; 51(7): 1284-7 Jaakson K, Zernant J, Külm M, Hutchinson A, Tõnisson N, Glavac D, Ravnik-Glavac M, Hawlina M, Meltzer M R, Caruso R C, Testa F, Maugeri A, Hoyng C B, Gouras P, Simonelli F, Lewis R A, Lupski J R, Cremers F P, Allikmets R. Genotyping Microarray (Gene Chip) for the ABCR (ABCA4) Gene. Hum Mutat 2003, 22: 395-403 Dawson E, Abecasis G R, Bumpstead S, Chen Y, Hunt S, Beare D M, Pabial J, Dibling T, Tinsley E, Kirby S, Carter D, Papaspyridonos M, Livingstone S, Ganske R, Lõhmussaar E, Zernant J, Tõnisson N, Remm M, Mägi R, Puurand T, Vilo J, Kurg A, Rice K, Deloukas P, Mott R, Metspalu A, Bentley D R, Cardon L R & Dunham I. A first-generation linkage disequilibrium map of human chromosome 22. Nature 2002; 418: 544-8 Gemignani F, Perra C, Landi S, Canzian F, Kurg A, Tõnisson N, Galanello R, Cao A, Metspalu A, Romeo G. Reliable detection of ß-thalassemia and G6PD mutations by a DNA microarray. Clin Chem 2002; 48(11): 2051-4 Lohmussaar, E; Zernant, J; Magi, R; Kepp, K; Puurand, T; Tonisson, N; Valvas, I; Slavin, G; Andreson, R; Kurg, A; Remm, M; Metspalu, A. SNP analysis and linkage disequilibrium map of the human chromosome 22 using APEX arrays. European Journal of Human Genetics, 10: 299-299 Suppl. 1 MAY 2002 Lohmussaar, E; Zernant, J; Mägi, R; Puurand, T; Tonisson, N; Valvas, I; Slavin, G; Kurg, A; Remm, M; Metspalu, A. SNP analysis and linkage disequilibrium map of the human chromosome 22 using Arrayed Primer EXtension (APEX) technology. American Journal of Human Genetics, 71 (4): 1633 Suppl. S OCT 2002 Roomere, H; Meiel, A; Tonisson, N; Zernant, J; Kurg, A; Pavel, H; Slavin, G; Hainaut, P; Metspalu, A Arrayed primer extension resequencing assay of TP53 tumour suppressor gene. European Journal of Human Genetics, 10: 292-292 Suppl. 1 MAY 2002 Tõnisson N , Zernant J, Kurg A, Pavel H, Slavin G, Roomere H, Meiel A, Hainaut P and Metspalu A. Evaluating the arrayed primer extension resequencing assay of TP53 tumor suppressor gene. Proc Natl Acad Sci USA 2002; 99(8): 5503-8 Metspalu, A; Zernant, J; Lohmussaar, E; Magi, R; Kepp, K; Puurand, T; Tonisson, N; Valvas, L; Pavel, H; Slavin, G; Andreson, R; Kurg, A; Remm, M. SNP analysis of the human chromosome 22 using APEX arrays. American Journal of Human Genetics, 69 (4): 1322 Suppl. 1 OCT 2001 Kurg, A; Lohmussaar, E; Nikopensius, T; Tonisson, N; Lushnikov, A; Kask, I; Zernant, J; Pavel, H; Metspalu, A. Arrayed Primer EXtension (APEX) as single nucleotide polymorphism (SNP) scoring technology. American Journal of Human Genetics, 67 (4): 1863 Suppl. 2 OCT 2000 Kurg A, Tõnisson N, Tollett J, Georgiou I, Shumaker J, Metspalu A. Arrayed Primer Extension: Solid phase four-color DNA resequencing and mutation detection technology. Genetic Testing 2000; 4(1): 1-7. Pavel H, Tõnisson N, Zernant J. Method for optimising the sequences of synthetic nucleic acids. (2000) Asper Biotech Ltd., Estonia. Application WO 02/42490 Tõnisson N, Kurg A, Kaasik K, Lõhmussaar E, Metspalu A. Unravelling genetic data by arrayed primer extension. Clinical Chemistry and Laboratory Medicine 2000; 38(2): 165-70 Tõnisson N, Kurg A, Lõhmussaar E, Metspalu A. Arrayed primer extension on the DNA chip – method and applications. Microarray Biochip Technologies (Ed. by M. Schena.) Biotechniques Books. Eaton Publishing, Natick MA USA 2000: 247-63 Kurg A, Tõnisson N, Metspalu A, Berik E. Laser diagnostic system for rapid mutation identification. Medical and Biological Engineering and Computing 1999; 37 suppl.1: 311 Metspalu A, Kaasik K, Tonisson N, Kurg A. Oligonucleotide array for mutation analysis in familial breast cancer. Disease Markers 1999; 15 (1-3): 117 Metspalu A, Saulep H, Kurg A, Tõnisson N. Primer extension from two-dimensional oligonucleotide grids for DNA sequence analysis. Genomis: Commercial Opportunities from a Scientific Revolution (Ed. by Dixon GK, Copping LG, Livingstone D), BIOS Scientific Publishers 1998: 217-9 |
viimati muudetud: 11.10.2005
| Curriculum Vitae (CV) | ||
| 1. | First Name | Neeme |
| 2. | Surname | Tõnisson |
| 3. | Institution | Institute of Molecular and Cell Biology University of Tartu, Estonia |
| 4. | Position | Senior Research Fellow |
| 5. | Date of birth | 17.02.1971 (day.month.year) |
| 6. | Education | 1989 – 1995 Medical Faculty, University of Tartu 1995 – 2000 Ph.D Student, University of Tartu |
| 7. | Research and professional experience |
1995 - 1996 Assistant, Women's Clinic, University of Tartu 1997 - 2004 Instructor of practical works in Molecular Biotechnology, Univ. of Tartu 1999 - 2001 Scientist, Asper, Ltd., Tartu, Estonia 2001 - 2002 Director of Genotyping Applications, Asper Biotech, Ltd. 2001 - 2002 Instructor, ESF international course "APEX on DNA Microarrays" 2003 - Senior Scientist, Asper Biotech, Ltd. 2004 Research Fellow, Institute of Molecular and Cell Biology, University of Tartu (in the group of Prof. M. Laan) 2004 - Visiting Scientist, Deutsches Krebsforschngszentrum (DKFZ), Heidelberg, Germany 2005 - Senior Research Fellow, Institute of Molecular and Cell Biology, University of Tartu |
| 8. | Academic degree | Ph.D (Molecular Diagnostics) |
| 9. | Dates and sites of earning the degrees |
University of Tartu, 2003 |
| 10. | Honours/awards | 2004 - Roman Herzog Research Fellowship, Alexander von Humboldt Foundation / Hertie Foundation |
| 11. | Research-administrative experience |
Member of the Estonian Society of Human Genetics 2000 Expert, Workgroup for the development of the Estonian Genome Project |
| 12. | Supervised dissertations |
Aune Ahman, MSc, 2003, superv. N. Tõnisson, A. Metspalu. Oligonukleotiidse kiibi valideerimine ja võrdlus teiste TP53 geeni uurimisel kasutatavate meetoditega. Tartu Ülikool Hanno Roomere, MSc, 2002, superv. N. Tõnisson, A. Metspalu. Praimerekstensioonil põhineva resekveneerimismeetodi väljatöötamine tuumorsupressorgeeni TP53 näitel. Tartu Ülikool |
| 13. | Current research program | a) development of method of Arrayed Primer Extension (APEX) for the genetic testing of various disorders and population genetic analyses b) analysis of genetic factors of arterial hypertension, comparative genetics analysis of the candidate genes (group of Prof. M.Laan) c) molecular diagnostics of cancer - mutation analysis (Univ. of Tartu, Asper Biotech) and epigenetic profiling (DKFZ) |
| 14. | Current grant funding | |
| 15. | List of most important publications |
Kringen P, Bergamaschi A, Undlien Due E, Wang Y, Menard S, Tagliabue E, Nesland JM, Ahman A, Tõnisson N, Børresen-Dale AL. Evaluation of Arrayed Primer Extension (APEX) in TP53 mutation detection in breast and ovarian carcinomas. Biotechniques 2005; 39(5):755-61 Le Calvez F, Ahman A, Tonisson N, Lambert J, Temam S, Brennan P, Zaridze DG, Metspalu A, Hainaut P. Arrayed Primer Extension Resequencing of Mutations in the TP53 Tumor Suppressor Gene: Comparison with Denaturing HPLC and Direct Sequencing. Clin Chem. 2005; 51(7): 1284-7 Jaakson K, Zernant J, Külm M, Hutchinson A, Tõnisson N, Glavac D, Ravnik-Glavac M, Hawlina M, Meltzer M R, Caruso R C, Testa F, Maugeri A, Hoyng C B, Gouras P, Simonelli F, Lewis R A, Lupski J R, Cremers F P, Allikmets R. Genotyping Microarray (Gene Chip) for the ABCR (ABCA4) Gene. Hum Mutat 2003, 22: 395-403 Dawson E, Abecasis G R, Bumpstead S, Chen Y, Hunt S, Beare D M, Pabial J, Dibling T, Tinsley E, Kirby S, Carter D, Papaspyridonos M, Livingstone S, Ganske R, Lõhmussaar E, Zernant J, Tõnisson N, Remm M, Mägi R, Puurand T, Vilo J, Kurg A, Rice K, Deloukas P, Mott R, Metspalu A, Bentley D R, Cardon L R & Dunham I. A first-generation linkage disequilibrium map of human chromosome 22. Nature 2002; 418: 544-8 Gemignani F, Perra C, Landi S, Canzian F, Kurg A, Tõnisson N, Galanello R, Cao A, Metspalu A, Romeo G. Reliable detection of ß-thalassemia and G6PD mutations by a DNA microarray. Clin Chem 2002; 48(11): 2051-4 Lohmussaar, E; Zernant, J; Magi, R; Kepp, K; Puurand, T; Tonisson, N; Valvas, I; Slavin, G; Andreson, R; Kurg, A; Remm, M; Metspalu, A. SNP analysis and linkage disequilibrium map of the human chromosome 22 using APEX arrays. European Journal of Human Genetics, 10: 299-299 Suppl. 1 MAY 2002 Lohmussaar, E; Zernant, J; Mägi, R; Puurand, T; Tonisson, N; Valvas, I; Slavin, G; Kurg, A; Remm, M; Metspalu, A. SNP analysis and linkage disequilibrium map of the human chromosome 22 using Arrayed Primer EXtension (APEX) technology. American Journal of Human Genetics, 71 (4): 1633 Suppl. S OCT 2002 Roomere, H; Meiel, A; Tonisson, N; Zernant, J; Kurg, A; Pavel, H; Slavin, G; Hainaut, P; Metspalu, A Arrayed primer extension resequencing assay of TP53 tumour suppressor gene. European Journal of Human Genetics, 10: 292-292 Suppl. 1 MAY 2002 Tõnisson N , Zernant J, Kurg A, Pavel H, Slavin G, Roomere H, Meiel A, Hainaut P and Metspalu A. Evaluating the arrayed primer extension resequencing assay of TP53 tumor suppressor gene. Proc Natl Acad Sci USA 2002; 99(8): 5503-8 Metspalu, A; Zernant, J; Lohmussaar, E; Magi, R; Kepp, K; Puurand, T; Tonisson, N; Valvas, L; Pavel, H; Slavin, G; Andreson, R; Kurg, A; Remm, M. SNP analysis of the human chromosome 22 using APEX arrays. American Journal of Human Genetics, 69 (4): 1322 Suppl. 1 OCT 2001 Kurg, A; Lohmussaar, E; Nikopensius, T; Tonisson, N; Lushnikov, A; Kask, I; Zernant, J; Pavel, H; Metspalu, A. Arrayed Primer EXtension (APEX) as single nucleotide polymorphism (SNP) scoring technology. American Journal of Human Genetics, 67 (4): 1863 Suppl. 2 OCT 2000 Kurg A, Tõnisson N, Tollett J, Georgiou I, Shumaker J, Metspalu A. Arrayed Primer Extension: Solid phase four-color DNA resequencing and mutation detection technology. Genetic Testing 2000; 4(1): 1-7. Pavel H, Tõnisson N, Zernant J. Method for optimising the sequences of synthetic nucleic acids. (2000) Asper Biotech Ltd., Estonia. Application WO 02/42490 Tõnisson N, Kurg A, Kaasik K, Lõhmussaar E, Metspalu A. Unravelling genetic data by arrayed primer extension. Clinical Chemistry and Laboratory Medicine 2000; 38(2): 165-70 Tõnisson N, Kurg A, Lõhmussaar E, Metspalu A. Arrayed primer extension on the DNA chip – method and applications. Microarray Biochip Technologies (Ed. by M. Schena.) Biotechniques Books. Eaton Publishing, Natick MA USA 2000: 247-63 Kurg A, Tõnisson N, Metspalu A, Berik E. Laser diagnostic system for rapid mutation identification. Medical and Biological Engineering and Computing 1999; 37 suppl.1: 311 Metspalu A, Kaasik K, Tonisson N, Kurg A. Oligonucleotide array for mutation analysis in familial breast cancer. Disease Markers 1999; 15 (1-3): 117 Metspalu A, Saulep H, Kurg A, Tõnisson N. Primer extension from two-dimensional oligonucleotide grids for DNA sequence analysis. Genomis: Commercial Opportunities from a Scientific Revolution (Ed. by Dixon GK, Copping LG, Livingstone D), BIOS Scientific Publishers 1998: 217-9 |
last updated: 11.10.2005
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