[ sulge aken ]
| Elulookirjeldus (CV) | ||
| 1. | Eesnimi | Tiina |
| 2. | Perekonnanimi | Alamäe |
| 3. | Töökoht | Tartu Ülikooli Molekulaar- ja Rakubioloogia Instituut |
| 4. | Ametikoht | Dotsent |
| 5. | Sünniaeg | 17.02.1955 (päev.kuu.aasta) |
| 6. | Haridus | kõrgem |
| 7. | Teenistuskäik | Tartu Ülikool, Taimefüsioloogia ja biokeemia kateeder, nooremteadur 1978-1984; assistent sealsamas 1984-1988; vanemõpetaja sealsamas 1988-1991; dotsent sealsamas 1991-1992; Molekulaar- ja Rakubioloogia Instituudis dotsent alates 1992. a. |
| 8. | Teaduskraad | Bioloogiakandidaat mikrobioloogia erialal |
| 9. | Teaduskraadi välja andnud asutus, aasta |
Valgevene TA Mikrobioloogia Instituut; 1986. |
| 10. | Tunnustused | Ülikooli teenetemedal, 2005. a. |
| 11. | Teadusorganisatsiooniline ja –administratiivne tegevus |
Eesti Mikrobioloogia Seltsi esimees (1994-2002); Euroopa Mikrobioloogia Seltside Liidu Eestipoolne esindaja; Geeni- ja Keskkonnatehnoloogia Tippkeskuse nõukogu liige; TÜ Bioloogia-Geograafiateaduskonna nõukogu liige |
| 12. | Juhendamisel kaitstud väitekirjad |
Helen Udras, PhD, 2005, juh. Tiina Alamäe. Hexose kinases and glucose transport in the yeast Hansenula polymorpha. Tartu Pille Pärn, MSc, 2003, juh. Tiina Alamäe. Pärmi Hansenula polymorpha maltaas. Tartu Silja Laht, MSc, 2001, juh. Tiina Alamäe. Pärmi Hansenula polymorpha glükokinaas: geeni kloneerimine ja valgu iseloomustamine. Tartu Toomas Kramarenko, MSc, 2000, juh. Tiina Alamäe. Heksoosi kinaaside roll suhkrute poolt põhjustatavas repressioonis pärmil Hansenula polymorpha. Tartu Lele Liiv, MSc, 1999, juh. Tiina Alamäe. Maltaasi iseloomustamine ja maltaasi geeni kloneerimine metülotroofsel pärmil Hansenula polymorpha. Tartu Helen Karp, MSc, 1998, juh. Tiina Alamäe. Heksoosi kinaasid ja glükoosi transport metülotroofsel pärmil Hansenula polymorpha. Tartu Aiki Järviste, MSc, 1995, juh. Tiina Alamäe. Ensüümiaktiivsuste määramine pärmi Pichia pinus permeabiliseeritud rakkudes. Tartu |
| 13. | Teadustöö põhisuunad | Glükoosi repressiooni mehhanismide uurimine metülotroofsetel pärmidel. Maltoosi ja sahharoosi kasutamise uurimine metülotroofsetel pärmidel. Uute biotehnoloogiliste ekspessioonisüsteemide väljatöötamine pärmidele ja bakteritele. |
| 14. | Jooksvad grandid | Uute mikroobsete süsteemide väljatöötamine ja testimine võõrvalkude ekspressiooniks. ETF grant 5676 (2004-2007); Osalen põhitäitjana ka sihtfinantseeritava teema 0182562Cs03 "Metaboolsete protsesside regulatsioon ja geneetilised ümberkorraldused mikroorganismides" alateemas C "Geeniekspressiooni regulatsiooni uurimine metülotroofsetel pärmidel" täitmisel. |
| 15. | Teaduspublikatsioonid |
Viigand K., Tammus K., Alamäe T. 2005. Clustering of MAL genes in Hansenula polymorpha: Cloning of the maltose permease gene and expression from the divergent intergenic region between the maltose permease and maltase genes. FEMS Yeast Res.; [Epub ahead of print] Karp H., Järviste A., Kriegel T. M., Alamäe T. 2004 Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha. Curr Genet., vol. 44, p. 268-76. Alamäe T., Pärn P., Viigand K., Karp H. (2003). Regulation of the Hansenula polymorpha maltase gene promoter in H. polymorpha and Saccharomyces cerevisiae. FEMS Yeast Res., vol. 4, p. 165-173. Laht S., Karp H., Kotka P., Järviste A., Alamäe T. 2002. Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae. Gene, vol. 296, p. 195-203. Liiv L., Pärn P., Alamäe T. 2001. Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Gene, vol. 265, p. 77-85. Kramarenko T., Karp H., Järviste A., Alamäe T. 2000. Sugar repression in the methylotrophic yeast Hansenula polymorpha studied by using hexokinase-negative, glucokinase-negative and double kinase-negative mutants. Folia Microbiol, vol. 45, p. 521-529. Alamäe T., Liiv, L. 1998. Glucose repression of maltase and methanol-oxidizing enzymes in the methylotrophic yeast Hansenula polymorpha: isolation and study of regulatory mutants. Folia Microbiol., vol. 43, p. 443-52. Karp H., Alamäe T. 1998. Glucose transport in a methylotrophic yeast Hansenula polymorpha. FEMS Microbiol Lett., vol. 66, p. 267-73. Truu J., Alamae T., Heinaru E., Talpsep E., Kokassaar U., Heinaru A., Tenno T. 1997. Impact of oil shale mine water on microbiological and chemical composition of north-eastern Estonian rivers - OIL SHALE, vol. 14, p. 526-532. Alamäe T., Järviste A. 1995. Permeabilization of methylotrophic yeasts Pichia pinus for intracellular enzyme alalysis: a quantitative study. J. Microbiol. Methods, vol. 22, p. 193-205. Alamäe T., Simisker J. 1994. Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression. Yeast, vol. 10, p. 1459-1466 Alamäe TO., Teugjas, KK., Soom, YO., Simisker, YA. 1985. Mutant of the methanol-assimilating yeast Pichia pinus partially resitant to catabolite repression. Microbiology, vol. 54, p. 512-517, |
viimati muudetud: 07.10.2005
| Curriculum Vitae (CV) | ||
| 1. | First Name | Tiina |
| 2. | Surname | Alamäe |
| 3. | Institution | Institute of Molecular and Cell Biology, University of Tartu |
| 4. | Position | Ass. Prof. |
| 5. | Date of birth | 17.02.1955 (day.month.year) |
| 6. | Education | higher |
| 7. | Research and professional experience |
University of Tartu, Dep. of Plant Physiology and Biochemistry, junior research scientist, 1978-1984; assistant, 1984-1988; lecturer, 1988-1991; ass. prof., since 1991. Ass. prof. of the Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, since 1992. |
| 8. | Academic degree | Ph D in microbiology |
| 9. | Dates and sites of earning the degrees |
Institute of Microbiology, Academy of Sciences of Belorussia, 1986. |
| 10. | Honours/awards | Medal of Tartu University, 2005. a. |
| 11. | Research-administrative experience |
Chairman of the Estonian Society for Microbiology (1994-2002). FEMS Delegate of Estonian Society for Microbiology. Member of the Board of the Centre of Excellence for Gene and Environmental Technology, University of Tartu and Estonian Biocentre. Member of the Council of the Department of Biology and Geography, University of Tartu |
| 12. | Supervised dissertations |
Helen Udras, PhD, 2005, superv. Tiina Alamäe. Hexose kinases and glucose transport in the yeast Hansenula polymorpha. Tartu Pille Pärn, MSc, 2003, superv. Tiina Alamäe. Pärmi Hansenula polymorpha maltaas. Tartu Silja Laht, MSc, 2001, superv. Tiina Alamäe. Pärmi Hansenula polymorpha glükokinaas: geeni kloneerimine ja valgu iseloomustamine. Tartu Toomas Kramarenko, MSc, 2000, superv. Tiina Alamäe. Heksoosi kinaaside roll suhkrute poolt põhjustatavas repressioonis pärmil Hansenula polymorpha. Tartu Lele Liiv, MSc, 1999, superv. Tiina Alamäe. Maltaasi iseloomustamine ja maltaasi geeni kloneerimine metülotroofsel pärmil Hansenula polymorpha. Tartu Helen Karp, MSc, 1998, superv. Tiina Alamäe. Heksoosi kinaasid ja glükoosi transport metülotroofsel pärmil Hansenula polymorpha. Tartu Aiki Järviste, MSc, 1995, superv. Tiina Alamäe. Ensüümiaktiivsuste määramine pärmi Pichia pinus permeabiliseeritud rakkudes. Tartu |
| 13. | Current research program | Genetics and molecular biology of glucose repression and glucose signaling in yeasts; Study of genetics and biochemistry of maltose and sucrose utilization in methylotrophic yeasts; Elaboration of new yeast and bacterial expression systems for biotechnology. |
| 14. | Current grant funding | 2004-2007. Elaboration of new microbial systems for the expression of foreign proteins. Grant No 5676 from Estonian Science Foundation. |
| 15. | List of most important publications |
Viigand K., Tammus K., Alamäe T. 2005. Clustering of MAL genes in Hansenula polymorpha: Cloning of the maltose permease gene and expression from the divergent intergenic region between the maltose permease and maltase genes. FEMS Yeast Res.; [Epub ahead of print] Karp H., Järviste A., Kriegel T. M., Alamäe T. 2004 Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha. Curr Genet., vol. 44, p. 268-76. Alamäe T., Pärn P., Viigand K., Karp H. (2003). Regulation of the Hansenula polymorpha maltase gene promoter in H. polymorpha and Saccharomyces cerevisiae. FEMS Yeast Res., vol. 4, p. 165-173. Laht S., Karp H., Kotka P., Järviste A., Alamäe T. 2002. Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae. Gene, vol. 296, p. 195-203. Liiv L., Pärn P., Alamäe T. 2001. Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Gene, vol. 265, p. 77-85. Kramarenko T., Karp H., Järviste A., Alamäe T. 2000. Sugar repression in the methylotrophic yeast Hansenula polymorpha studied by using hexokinase-negative, glucokinase-negative and double kinase-negative mutants. Folia Microbiol, vol. 45, p. 521-529. Alamäe T., Liiv, L. 1998. Glucose repression of maltase and methanol-oxidizing enzymes in the methylotrophic yeast Hansenula polymorpha: isolation and study of regulatory mutants. Folia Microbiol., vol. 43, p. 443-52. Karp H., Alamäe T. 1998. Glucose transport in a methylotrophic yeast Hansenula polymorpha. FEMS Microbiol Lett., vol. 66, p. 267-73. Truu J., Alamae T., Heinaru E., Talpsep E., Kokassaar U., Heinaru A., Tenno T. 1997. Impact of oil shale mine water on microbiological and chemical composition of north-eastern Estonian rivers - OIL SHALE, vol. 14, p. 526-532. Alamäe T., Järviste A. 1995. Permeabilization of methylotrophic yeasts Pichia pinus for intracellular enzyme alalysis: a quantitative study. J. Microbiol. Methods, vol. 22, p. 193-205. Alamäe T., Simisker J. 1994. Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression. Yeast, vol. 10, p. 1459-1466 Alamäe TO., Teugjas, KK., Soom, YO., Simisker, YA. 1985. Mutant of the methanol-assimilating yeast Pichia pinus partially resitant to catabolite repression. Microbiology, vol. 54, p. 512-517, |
last updated: 07.10.2005
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