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Elulookirjeldus (CV)
1.Eesnimi Tuuli
2.Perekonnanimi Käämbre
3.Töökoht Keemilise ja Bioloogilise Füüsika Instituut
4.Ametikoht teadur
5.Sünniaeg 20.06.1960 (päev.kuu.aasta)
6.Haridus Meditsiinidoktor
7.Teenistuskäik 1983-1986 Keemia Instituut, vaneminsener
1986-1991 Väikeettevõte „Kemotex”, keemik
1991-1995 Keemilise ja Bioloogilise Füüsika Instituut, vaneminsener
1996- Keemilise ja Bioloogilise Füüsika Instituut, teadur
8.Teaduskraad Meditsiinidoktor
9.Teaduskraadi välja
andnud asutus, aasta		 2004 Tartu Ülikool, Arstiteduskond
10.Tunnustused
11.Teadusorganisatsiooniline
ja –administratiivne
tegevus
12.Juhendamisel kaitstud
väitekirjad
13.Teadustöö põhisuunad Peamisteks uurimisvaldkondadeks on südame ja skeletilihaste energia metabolismi regulatsiooni uurimine in vivo tingimustes ning ning mitokondrite ja tsütoskeleti vaheliste interaktsioonide funktsionaalse tähtsuse selgitamine.
14.Jooksvad grandid ETF nr. 6142 "Kompartmentaliseeritud energiatransporgi võrgud kardiomüotsüütides ja eelkohastumise mehhanismid"(põhitäitja)
ETF nr. 5515 "Mitokondrite ja ATPaaside funktsionaalsed kompleksid kui energeetilise metabolismi põhiüksused oksüdatiivsetes lihasrakkudes"(põhitäitja)
15.Teaduspublikatsioonid

. Anmann T., Eimre M, Kuznetsov A.V., Andrienko T., Kaambre T., Sikk P., Seppet E., Tiivel T., Vendelin M., Seppet E. and Saks V.A. (2005) Calcium-induced contraction of sarcomeres changes regulation of mitochondrial respiration in permeabilized cardiac cells. FEBS Journal 272, 3145-3161.

Seppet E. K., Margus Eimre, Tiia Anmann, Evelin Seppet, Nadezhda Peet, Tuuli Käämbre, Kalju Paju, Andres Piirsoo, Andrei V. Kuznetsov, Marko Vendelin, Frank N. Gellerich, Stephan Zierz, Valdur A. Saks (2005) Intracellular energetic units in healthy and diseased hearts, Experimental and Clinical Cardiology 10, 173-183.

Kulp, M., Kaljurand, M., Kaambre, T., Sikk, P., Saks, V. (2004) In situ monitoring of kinetics of metabolic conversion of ATP to ADP catalyzed by MgATPases of muscle Gastrocnemius skinned fibers using micellar electrokinetic chromatography. Electrophoresis. 17: 2996-3002

Seppet, E. K., Eimre, M., Andrienko, T., Kaambre, T., Sikk, P., Kuznetsov, A. V., Saks, V. (2004) Studies of mitochondrial respiration in muscle cells in situ: Use and misuse of experimental evidence in mathematical modeling Mol. Cell. Biochem. 256: 219–227.

Andrienko, T. Kuznetsov, A., V., Kaambre, T., Usson, Y., Orosco, A., Appaix, F., Tiivel, T., Sikk, P., Vendelin, M., Margreiter, R., Saks, V. A. (2003) Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells J. Exp. Biol. 206: 2059-72.

Appaix, F., Kuznetsov, A. V., Usson, Y., Kay, L., Andrienko, T., Olivares, J., Kaambre,T., Sikk, P., Margreiter, R., Saks, V. .(2003) Possible role of cytoskeleton in intracellular arrangement and regulation of mitochondria. Exp. Physiol. 88: 175-90.

Saks, V., Kuznetsov, A., Andrienko, T., Usson, Y., Appaix, F., Guerrero, K., Kaambre, T., Sikk, P., Lemba, M., Vendelin, M. (2003) Heterogeneity of ADP diffusion and regulation of respiration in cardiac cells. Biophys J. 84: 3436-56.

Appaix, F., Guerrero, K., Rampal, D., Izikki, M., Kaambre, T., Sikk, P., Brdiczka, D., Riva-Lavieille, C., Olivares, J., Longuet, M., Antonsson, B., Saks VA. (2002) Bax and heart mitochondria: uncoupling and inhibition of respiration without permeability transition. Biochim. Biophys. Acta.; 1556: 155-67

Seppet, E. K., Kadaja, L., Braun, U., Kisand, K., Metskula, K., Uibo, R., Kaambre, T., Saks, V. A. (2002) Intracellular energetic units in cardiac cells: Targets in primary biliary cirrhosis, J. Mol. Cell. Cardiol., 34: A57.

Saks, V. A., Kaambre, T., Sikk, P., Eimre, M., Orlova, E., Paju, K., Piirsoo, A., Appaix, F., Kay, L., Regitz-Zagrosek, V., Fleck, E., Seppet, E. (2001) Intracellular energetic units in red muscle cells Biochem. J. 356: 643-657

Seppet, E. K., Kaambre, T., Sikk, P., Tiivel, T., Vija, H., Tonkonogi, M., Sahlin, K., Kay, L., Appaix, F., Braun, U., Eimre, M., Saks, V. A. (2001) Functional complexes of mitochondria with Ca,MgATPases of myofibrils and sarcoplasmic reticulum in muscle cells. Biochim. Biophys. Acta; 1504: 379-95.

Tiivel, T., Kadaya, L., Kuznetsov, A., Kaambre, T., Peet, N., Sikk, P., Braun, U., Ventura-Clapier, R., Saks, V., Seppet, E. K. (2000) Developmental changes in regulation of mitochondrial respiration by ADP and creatine in rat heart in vivo. Mol. Cell. Biochem. 208: 119-28.

Kaambre, T., Tougu, V., Kaambre, P., Vija, H., Sikk, P. (1999) Hydrolysis of emulsified mixtures of triacylglycerols by pancreatic lipase. Biochim. Biophys. Acta. 1431: 97-106.

Seppet, E. K., Kadaya, L., Peet, N., Käämbre, T., Sikk, P., Saks, V. A. (1998) The 27.5 kD protein appears to control the oxidative phosphorylation in slow twitch muscle cells, Pathophysiology, 5: 121.

Kay, L., Li, Z., Mericskay, M., Olivares, J., Tranqui, L., Fontaine, E., Tiivel, T., Sikk, P., Kaambre, T., Samuel, J. L., Rappaport, L., Usson, Y., Leverve, X., Paulin, D., Saks, V. A. (1997) Study of regulation of mitochondrial respiration in vivo. An analysis of influence of ADP diffusion and possible role of cytoskeleton. Biochim. Biophys. Acta. 1322: 41-59.

Parve, O., Vallikivi, I., Metsala, A., Lille, Ü., Tõugu, V., Sikk, P., Käämbre, T., Vija H., Pehk, T. (1997) Lipase-catalysed enantioselective hydrolysis: Interpretation of the kinetic results in terms of frontier orbital localisation, Tetrahedron 53: 4889-4900.

1. Kuznetsov, A. V., Tiivel, T., Sikk, P., Kaambre, T., Kay, L., Daneshrad, Z., Rossi, A., Kadaja, L., Peet, N., Seppet, E., Saks, V. A. (1996) Striking differences between the kinetics of regulation of respiration by ADP in slow-twitch and fast-twitch muscles in vivo. Eur. J. Biochem. 241: 909-15.

viimati muudetud: 10.10.2005

Curriculum Vitae (CV)
1.First Name Tuuli
2.Surname Käämbre
3.Institution Institute of Chemical Physics and Biophysics
4.Position res. Scientist
5.Date of birth 20.06.1960 (day.month.year)
6.Education Dr.Med
7.Research and
professional experience		 1983-1986 Institute of Chemistry, senior engineer
1986-1991 Small enterprice „Kemotex”, chemist
1991-1995 Institute of Chemical Physics and Biophysics, senior engineer
1996- Institute of Chemical Physics and Biophysics, res. Scientist
8.Academic degree Dr.Med.
9.Dates and sites of
earning the degrees		 University of Tartu, Faculty of Medicine
10.Honours/awards
11.Research-administrative
experience
12.Supervised dissertations
13.Current research program The subjects of my researc activities have been the regulation of cardiac and skeletal muscle energy metabolism in vivo, energy networks in muscle cells and studys of functional significance of mitochondria-cytosceleton interactions
14.Current grant funding ESF no. 6142 "Compartmentalized energy transfer in cardiomyocytes and mechanisms of preconditioning"
ESF no. 5515 "Functional complexes of mitochondria with ATPases as the principal units of energetical metabolism in oxidative muscle cells"
15.List of most important publications

. Anmann T., Eimre M, Kuznetsov A.V., Andrienko T., Kaambre T., Sikk P., Seppet E., Tiivel T., Vendelin M., Seppet E. and Saks V.A. (2005) Calcium-induced contraction of sarcomeres changes regulation of mitochondrial respiration in permeabilized cardiac cells. FEBS Journal 272, 3145-3161.

Seppet E. K., Margus Eimre, Tiia Anmann, Evelin Seppet, Nadezhda Peet, Tuuli Käämbre, Kalju Paju, Andres Piirsoo, Andrei V. Kuznetsov, Marko Vendelin, Frank N. Gellerich, Stephan Zierz, Valdur A. Saks (2005) Intracellular energetic units in healthy and diseased hearts, Experimental and Clinical Cardiology 10, 173-183.

Kulp, M., Kaljurand, M., Kaambre, T., Sikk, P., Saks, V. (2004) In situ monitoring of kinetics of metabolic conversion of ATP to ADP catalyzed by MgATPases of muscle Gastrocnemius skinned fibers using micellar electrokinetic chromatography. Electrophoresis. 17: 2996-3002

Seppet, E. K., Eimre, M., Andrienko, T., Kaambre, T., Sikk, P., Kuznetsov, A. V., Saks, V. (2004) Studies of mitochondrial respiration in muscle cells in situ: Use and misuse of experimental evidence in mathematical modeling Mol. Cell. Biochem. 256: 219–227.

Andrienko, T. Kuznetsov, A., V., Kaambre, T., Usson, Y., Orosco, A., Appaix, F., Tiivel, T., Sikk, P., Vendelin, M., Margreiter, R., Saks, V. A. (2003) Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells J. Exp. Biol. 206: 2059-72.

Appaix, F., Kuznetsov, A. V., Usson, Y., Kay, L., Andrienko, T., Olivares, J., Kaambre,T., Sikk, P., Margreiter, R., Saks, V. .(2003) Possible role of cytoskeleton in intracellular arrangement and regulation of mitochondria. Exp. Physiol. 88: 175-90.

Saks, V., Kuznetsov, A., Andrienko, T., Usson, Y., Appaix, F., Guerrero, K., Kaambre, T., Sikk, P., Lemba, M., Vendelin, M. (2003) Heterogeneity of ADP diffusion and regulation of respiration in cardiac cells. Biophys J. 84: 3436-56.

Appaix, F., Guerrero, K., Rampal, D., Izikki, M., Kaambre, T., Sikk, P., Brdiczka, D., Riva-Lavieille, C., Olivares, J., Longuet, M., Antonsson, B., Saks VA. (2002) Bax and heart mitochondria: uncoupling and inhibition of respiration without permeability transition. Biochim. Biophys. Acta.; 1556: 155-67

Seppet, E. K., Kadaja, L., Braun, U., Kisand, K., Metskula, K., Uibo, R., Kaambre, T., Saks, V. A. (2002) Intracellular energetic units in cardiac cells: Targets in primary biliary cirrhosis, J. Mol. Cell. Cardiol., 34: A57.

Saks, V. A., Kaambre, T., Sikk, P., Eimre, M., Orlova, E., Paju, K., Piirsoo, A., Appaix, F., Kay, L., Regitz-Zagrosek, V., Fleck, E., Seppet, E. (2001) Intracellular energetic units in red muscle cells Biochem. J. 356: 643-657

Seppet, E. K., Kaambre, T., Sikk, P., Tiivel, T., Vija, H., Tonkonogi, M., Sahlin, K., Kay, L., Appaix, F., Braun, U., Eimre, M., Saks, V. A. (2001) Functional complexes of mitochondria with Ca,MgATPases of myofibrils and sarcoplasmic reticulum in muscle cells. Biochim. Biophys. Acta; 1504: 379-95.

Tiivel, T., Kadaya, L., Kuznetsov, A., Kaambre, T., Peet, N., Sikk, P., Braun, U., Ventura-Clapier, R., Saks, V., Seppet, E. K. (2000) Developmental changes in regulation of mitochondrial respiration by ADP and creatine in rat heart in vivo. Mol. Cell. Biochem. 208: 119-28.

Kaambre, T., Tougu, V., Kaambre, P., Vija, H., Sikk, P. (1999) Hydrolysis of emulsified mixtures of triacylglycerols by pancreatic lipase. Biochim. Biophys. Acta. 1431: 97-106.

Seppet, E. K., Kadaya, L., Peet, N., Käämbre, T., Sikk, P., Saks, V. A. (1998) The 27.5 kD protein appears to control the oxidative phosphorylation in slow twitch muscle cells, Pathophysiology, 5: 121.

Kay, L., Li, Z., Mericskay, M., Olivares, J., Tranqui, L., Fontaine, E., Tiivel, T., Sikk, P., Kaambre, T., Samuel, J. L., Rappaport, L., Usson, Y., Leverve, X., Paulin, D., Saks, V. A. (1997) Study of regulation of mitochondrial respiration in vivo. An analysis of influence of ADP diffusion and possible role of cytoskeleton. Biochim. Biophys. Acta. 1322: 41-59.

Parve, O., Vallikivi, I., Metsala, A., Lille, Ü., Tõugu, V., Sikk, P., Käämbre, T., Vija H., Pehk, T. (1997) Lipase-catalysed enantioselective hydrolysis: Interpretation of the kinetic results in terms of frontier orbital localisation, Tetrahedron 53: 4889-4900.

1. Kuznetsov, A. V., Tiivel, T., Sikk, P., Kaambre, T., Kay, L., Daneshrad, Z., Rossi, A., Kadaja, L., Peet, N., Seppet, E., Saks, V. A. (1996) Striking differences between the kinetics of regulation of respiration by ADP in slow-twitch and fast-twitch muscles in vivo. Eur. J. Biochem. 241: 909-15.

last updated: 10.10.2005

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