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| Elulookirjeldus (CV) | ||
| 1. | Eesnimi | Peep |
| 2. | Perekonnanimi | Palumaa |
| 3. | Töökoht | Tallinna Tehnikaülikool, Geenitehnoloogia Instituut |
| 4. | Ametikoht | professor |
| 5. | Sünniaeg | 30.06.1959 (päev.kuu.aasta) |
| 6. | Haridus | 1986 Ph.D. (teaduste kandidaadi kraad orgaanilises keemias) 1982-1986 Tartu Ülikool, orgaanilise keemia kateeder, aspirant 1977-1982 Tartu Ülikool, keemiaosakond, diplom cum laude bioorgaanilises keemias |
| 7. | Teenistuskäik | 2002 sept.- k.a.: TTÜ Geenitehnoloogia Instituudi genoomika ja proteoomika korraline professor 2001-2002: TTÜ Geenitehnoloogia Keskus, vanemteadur 1999-2001: TTÜ Alus- ja Rakenduskeemia Instituut, vanemteadur 1994 dets.-1999 sept: TÜ Molekulaar- ja Rakubioloogia Instituut, biokeemia korraline professor 1993 sept. TÜ Molekulaar- ja Rakubioloogia Instituut, biokeemia ôppetooli professori kt. 1992 sept.-1993 veebr: Tartu Ülikool, Bioorgaanilise keemia laboratoorium, vanemteadur 1991-1992: Zürichi Ülikool, Biokeemia Instituut, postdoktorant / vanemassistent 1990 - 1991: Zürichi Ülikool, Biokeemia Instituut, postdoktorant / assistent 1989 - 1990: Zürichi Ülikool, Biokeemia Instituut, Sveits, postdoktorant 1989 mai: Kuopio Ülikool, Soome, külalisteadur 1986-1989: TÜ Bioorgaanilise keemia laboratoorium, nooremteadur, teadur ja vanemteadur. |
| 8. | Teaduskraad | teaduste kandidaat (Ph.D.) |
| 9. | Teaduskraadi välja andnud asutus, aasta |
Eesti Teaduste Akadeemia Keemiainstituut |
| 10. | Tunnustused | |
| 11. | Teadusorganisatsiooniline ja –administratiivne tegevus |
Eesti Biokeemia Selts, viitsepresident 2002- EL 5 Raamprogrammi projekti: The role of zinc metabolism in Alzheimer’s disease. Elaboration of a new strategy for prevention and therapy of Alzheimer’s disease" koordinaator 2000–2002 Swiss Science Officer – Šveitsiga teaduskoostöö organisaator Tallinna Tehnikaülikoolis 2000–2002 Swiss Science Officer at Tallinn University of Technology 1995-1998 EÜ TEMPUS JEP projekti “Restructuring of Biochemical Education in the University of Tartu” koordinaator. |
| 12. | Juhendamisel kaitstud väitekirjad |
Elo Eriste, PhD, 2004, juh. Peep Palumaa. Purification, structure and function of bioactive peptides. Karolinska Instituut, Rootsi Indrek Tammiste, MSc, 2004, juh. Peep Palumaa. Metallotioneiin-2 ja metallotioneiin-3 metallisidumisomaduste ja sekundaarstruktuuri võrdlev analüüs. Tallinna Tehnikaülikool Julia Smirnova, MSc, 2004, juh. Peep Palumaa. Metallotioneiini isovormide metallide sidumisomaduste võrdlev analüüs. Tallinna Tehnikaülikool Olga Njunkova, MSc, 2002, juh. Peep Palumaa. Metallotioneiin-3 metallide sidumisomaduste uuringud ESI-MS abil. Tallinna Tehnikaülikool Liina Kangur, MSc, 2000, juh. Peep Palumaa. Metallotioneiin-ligand seostumiskonstantide määüramine. Tartu Ülikool Maret Vaher, MSc, 1995, juh. Peep Palumaa. Kaadmium-metallotioneiini reaktsioon vask(II)-ga. Tartu Ülikool Rain Lehtme, DSc, 1995, juh. Peep Palumaa. Inimese ajuspetsiifilise metallotioneiini ekspresseerimine E. colis. Tartu Ülikool |
| 13. | Teadustöö põhisuunad | Metalloproteiinide struktuursed ja spektroskoopilised uuringud (NMR-, EXAFS-, UV-, CD-, luminestsents-, aatomabsorbtsioon-, röntgendifraktsioon, ESI TOF mass-spektroskoopia jne.) metalloproteiinide funktsionaalsed uuringud (valk – valk, valk – ligand valk – metall interaktsioonid) Rekombinantsete peptiidide ja valkude biotehnoloogia Tsingi ja vase metabolism normaalses ja patoloogilistes tingimustes Alzheimeri haiguse uute terapeutikumide väljatöötamine. |
| 14. | Jooksvad grandid | 1. EL 5. Raamprogrammi CA projekt: The role of zinc metabolism in Alzheimer’s disease. Elaboration of a new strategy for prevention and therapy of Alzheimer’s disease" Koordinaator 2. DFG & University of Münster “Structure of polynuclear metal thiolate clusters”. TTÜ & Münsteri Ülikooli koostöögrant 3. "Structural studioes of metallothionein-4" Gebert Rüf Stiftung, Sveits, koostöögrant Zürichi Ülikooli Biokeemia Instituudiga 4. ETF grant 5635 "Tsingi metabolismi roll Alzheimeri haiguses. Uue strateegia väljatöötamine Alzheimeri haiguse preventsiooniks ja raviks" |
| 15. | Teaduspublikatsioonid |
Palumaa, P., Tammiste, I., Kangur, L., Kruusel, K., Jörnvall, H., Sillard, R. Metal-binding of metallothionein-3 versus metallothionein-2: lower affinity and higher plasticity (2005), BBA - Proteins and Proteomics, 1747 (2), 205-211. Palumaa, P., Voronova, A., Kangur, L., Sillard, R., Meyer-Klauke, W., Meyer, T., Rompel, A. „Mammalian copper chaperone Cox17 exists in two metalloforms linked by oxydative switch“. The FEBS Journal 272, Supplement 1, July 2005. Abstracts 30th FEBS Congress and 9th IUMB Conference, Budapest 2-7 July 2005, p. 386-387 Palumaa, P. Kangur, L. Voronova, A. and Sillard, R. Mechanism of metal binding by Cox17, a copper chaperone for cytochrome c oxidase. (2004), Biochem. J. 382, 307-314. Eriste E, Kruusel K, Palumaa P, Jörnvall H, Sillard R. Purification of recombinant human apometallothionein-3 and reconstitution with zinc. Protein Expr. Purif. 2003, 31(1), 161-165. Palumaa P, Eriste E, Kangur L, Jörnvall H, Sillard R. Metal binding to brain-specific metallothionein-3 studied by electrospray ionization mass spectrometry. Cell. Mol. Biol. 2003, 49(5), 763-768. Palumaa, P., Eriste, E., Njunkova, O., Pokras, L., Jörnvall, H., and Sillard, R. The brain-specific metallothionein-3 has higher metal-binding capacity as compared to the ubiquitous metallothioneins and binds metals non-cooperatively. (2002) Biochemistry, 41(19), 6158-6163. Palumaa, P., Njunkova, O., Pokras, L., Eriste, E., Jörnvall, H., and Sillard, R. Evidence for non-isostructural replacement of Zn2+ with Cd2+ in the b-domain of brain-specific metallothionein-3. (2002) FEBS Letters, 527, 76 - 80. Kangur, L and Palumaa, P. Reactivity of Metallothionein towards 5,5'-dithio-bis(2-nitrobenzoic acid). New Method for determination of binding constants for metallothionein – ligand interactions. (2001) Eur. J. Biochem., 268, 4979-4987. Vaher, M., Romero-Isart, N., Vasak, M. and Palumaa, P. Reactivity of Cd7-metallothionein with Cu(II) ions: Evidence for a cooperative formation of Cd3,Cu(I)5-metallothionein (2001) J. Inorg. Biochem. 83, 1-6. Bonetto, V., Kangur, L., Palumaa P., Mutt V., Jörnvall H., Sillard R. Large scale purification of Calbindin D9K from Porcine Intestine. (1999) Protein Expr. Purif. 17, 387 - 391. Palumaa, P. and Lehtme, R. Biochemical characterization of monomeric and dimeric cadmium-substituted metallothionein-3. In: Metallothionein IV. 1998 Birkhäuser, p. 63 – 69. Palumaa, P. and Vaher, M. Metal-induced dimerization of Cd7-metallothionein. Role of Anions. (1996) Ann. Clin. Lab. Sci. Vol. 26, No. 3, 264-268. Palumaa P., Vasak M. Formation and spectroscopic characterization of a novel monomeric cadmium- and phosphate-containing form of metallothionein. (1993) Biochemistry 32, 2874-2879. Palumaa P., Mackay E. A., Vasak M. Nonoxidative cadmium-dependent dimerization of Cd7-metallothionein from rabbit liver. (1992) Biochemistry 31, 2181-2186. Palumaa P., Vasak M. Binding of inorganic phosphate to the cadmium-induced dimeric form of metallothionein from rabbit liver. (1992) Eur. J. Biochem. 205, 1131-1135. Santa H., Laatikainen R., Lötjonen S., Palumaa P. Dynamic NMR determination of the N,N-dimethyl inversion rate and apparent Inversion free nergy in 2-hydroxy-N,N-dimethyl-2-phenylethylamine. (1992) J. Chem. Research (S), 198-199. Zorko M., Palumaa P., Kask K. Binding of some aromatic ligands to the active site of acetylcholinesterase. (1991) Periodicum Biologorum 2, 355-356. Zorko M., Langel U., Palumaa P. Simultaneous action of quaternary ammonium ligands and alkanesulfonylchlorides on electric eel acetylcholinesterase. (1990) Periodicum Biologorum 4, 385-391. Palumaa P., Kask K. Biomimetic modelling of spatial structure of acetylcholinesterase active site with cyclodextrins. (1989) Organic Reactivity 26, 122-141. Sepp A., Palumaa P., Langel U., Järv J., Zorko M. Spatial orientation of n-alkanesulfonyl chlorides in the active center of cholinesterases. (1989) Bioorg. Chem. 17, 79-85. Eller M., Järv J., Palumaa P. Influence of non-radioactive ligand on kinetics of N-methyl-[3H]scopolamine binding to muscarinic receptor. (1988) Organic reactivity 25, 372-386. Palumaa P., Järv J., Schevchenko V., Myasojedov N. Synthesis f -(N,N-dimethylamino)4,-3H-acetophenon. (1986) Radiokhim.(USSR) 22, 231-232. Palumaa P., Järv J. Alkylboronic acid acceleration of acetylcholinesterase alkylation with N,N- dimethyl-2-phenylaziridinium ion. (1985) Bioorg. Khim. (USSR) 11, 1210-1216. Palumaa P., Raidaru G., Schevchenko V., Järv J., Myasojedov N Synthesis of tritium labeled N,N- dimethyl-2-phenylaziridinium and its reaction with acetylcholinesterase. (1985) Bioorg. Khim. (USSR) 11, 1348-1352. Palumaa P., Soomets U., Järv J. Effects of reaction medium and temperature on solvolysis of N,N-dimethyl-2-phenylaziridinium ion. (1985) Organic reactivity 22, 238-257. Palumaa P., Järv J. Alkylboronic acids accelerate affinity labeling of acetylcholinesterase with N,N- dimethyl-2-phenylaziridinium ion. (1984) Biochim. Biophys. Acta 784, 35-39. Palumaa P., Raba R., Järv J. Site-specificity of butyrylcholinesterase alkylation with N,N- dimethyl-2- phenylaziridinium ion. (1984) Biochim. Biophys. Acta 791, 15-20. Palumaa P., Käämbre T., Järv J. Kinetics of the reaction of N,N-dimethyl-2-phenylaziridinium ions with muscarinic receptor and acetylcholinesterases. (1983) Bioorg. Khim. (USSR) 9, 1348-1355. Palumaa P., Mähar A,. Järv J. Kinetic analysis of butyrylcholinesterase inhibition with N,N- dimethyl-2-phenylaziridinium ion. (1982) Bioorg. Chem. 11, 394-403. |
viimati muudetud: 15.08.2005
| Curriculum Vitae (CV) | ||
| 1. | First Name | Peep |
| 2. | Surname | Palumaa |
| 3. | Institution | Tallinn University of Technology , Department of Gene Technology |
| 4. | Position | professor |
| 5. | Date of birth | 30.06.1959 (day.month.year) |
| 6. | Education | 1986 Candidate of sciences (Ph.D.), Institute of Chemistry, Estonian Academy of Sciences 1977-1982 Diploma Cum Laude (Bioorganic Chemistry), University of Tartu |
| 7. | Research and professional experience |
2002-present Professor of genomics and proteomics, Institute of Gene Technology, Tallinn Technical University, Estonia 2001-2002Senior Research Scientist, Center of Gene Technology, Tallinn Technical University, Estonia 1999-2001 Senior Research Scientist, Institute of Basic and Applied Chemistry, Tallinn Technical University, Estonia 1993-1999 Professor of Biochemistry, Institute of Molecular and Cell Biology, University of Tartu, Estonia 1996 Visiting Research Scientist, Institute of Biochemistry, University of Zürich 1995 Visiting Research Scientist, Institute of Chemistry, University of Münster, Germany 1992 – 1993 Senior Research Scientist, Laboratory of Bioorganic Chemistry, University of Tartu, Estonia 1991 – 1992 Senior Assistant, Institute of Biochemistry, Univesity of Zürich, Switzerland 1990 – 1991 Assistant, Institute of Biochemistry, Univesity of Zürich 1991 – 1992 Postdoctoral Fellow, Institute of Biochemistry, Univesity of Zürich 1986 – 1989 Research Scientist, Laboratory of Bioorganic Chemistry, University of Tartu |
| 8. | Academic degree | candidate of sciences (Ph.D.) |
| 9. | Dates and sites of earning the degrees |
Institute of Chemistry, Estonian Academy of Sciences |
| 10. | Honours/awards | |
| 11. | Research-administrative experience |
Estonian Biochemical Society, vice president 2002-2005 EU 5th FP Concerted action project ”Role of zinc in Alzheimer’s disease. Elaboration of new strategy for prevention and therapy of Alzheimer’s disease”. Coordinator 1995–1998 EU TEMPUS JEP “Restructuring of biochemical education at the University of Tartu” Coordinator |
| 12. | Supervised dissertations |
Elo Eriste, PhD, 2004, superv. Peep Palumaa. Purification, structure and function of bioactive peptides. Karolinska Instituut, Rootsi Indrek Tammiste, MSc, 2004, superv. Peep Palumaa. Metallotioneiin-2 ja metallotioneiin-3 metallisidumisomaduste ja sekundaarstruktuuri võrdlev analüüs. Tallinna Tehnikaülikool Julia Smirnova, MSc, 2004, superv. Peep Palumaa. Metallotioneiini isovormide metallide sidumisomaduste võrdlev analüüs. Tallinna Tehnikaülikool Olga Njunkova, MSc, 2002, superv. Peep Palumaa. Metallotioneiin-3 metallide sidumisomaduste uuringud ESI-MS abil. Tallinna Tehnikaülikool Liina Kangur, MSc, 2000, superv. Peep Palumaa. Metallotioneiin-ligand seostumiskonstantide määüramine. Tartu Ülikool Maret Vaher, MSc, 1995, superv. Peep Palumaa. Kaadmium-metallotioneiini reaktsioon vask(II)-ga. Tartu Ülikool Rain Lehtme, DSc, 1995, superv. Peep Palumaa. Inimese ajuspetsiifilise metallotioneiini ekspresseerimine E. colis. Tartu Ülikool |
| 13. | Current research program | Structural proteomics, neurodegenerative diseases, biomedical and environmental applications of metalloproteins, Bioinorganic chemistry of zinc and copper, structural and spectroscopic studies of metalloproteins Biomimetic modeling and mechanisms of enzyme action Quantitative structure - affinity relationships in enzymatic reactions, affinity labeling of cholinesterases, synthesis of tritium – labeled ligands |
| 14. | Current grant funding | 2002 - 2005 EU 5th FP Concerted action project ”Role of zinc in Alzheimer’s disease. Elaboration of new strategy for prevention and therapy of Alzheimer’s disease”. Coordinator 2002 - 2006 Collaboration Grant with University of Zürich from Gebert Rüf Stiftung 1994 - 2002 Estonian Science Foundation Grants "Role of zinc in Alzheimer’s disease. Elaboration of new strategy for prevention and therapy of Alzheimer’s disease” |
| 15. | List of most important publications |
Palumaa, P., Tammiste, I., Kangur, L., Kruusel, K., Jörnvall, H., Sillard, R. Metal-binding of metallothionein-3 versus metallothionein-2: lower affinity and higher plasticity (2005), BBA - Proteins and Proteomics, 1747 (2), 205-211. Palumaa, P., Voronova, A., Kangur, L., Sillard, R., Meyer-Klauke, W., Meyer, T., Rompel, A. „Mammalian copper chaperone Cox17 exists in two metalloforms linked by oxydative switch“. The FEBS Journal 272, Supplement 1, July 2005. Abstracts 30th FEBS Congress and 9th IUMB Conference, Budapest 2-7 July 2005, p. 386-387 Palumaa, P. Kangur, L. Voronova, A. and Sillard, R. Mechanism of metal binding by Cox17, a copper chaperone for cytochrome c oxidase. (2004), Biochem. J. 382, 307-314. Eriste E, Kruusel K, Palumaa P, Jörnvall H, Sillard R. Purification of recombinant human apometallothionein-3 and reconstitution with zinc. Protein Expr. Purif. 2003, 31(1), 161-165. Palumaa P, Eriste E, Kangur L, Jörnvall H, Sillard R. Metal binding to brain-specific metallothionein-3 studied by electrospray ionization mass spectrometry. Cell. Mol. Biol. 2003, 49(5), 763-768. Palumaa, P., Eriste, E., Njunkova, O., Pokras, L., Jörnvall, H., and Sillard, R. The brain-specific metallothionein-3 has higher metal-binding capacity as compared to the ubiquitous metallothioneins and binds metals non-cooperatively. (2002) Biochemistry, 41(19), 6158-6163. Palumaa, P., Njunkova, O., Pokras, L., Eriste, E., Jörnvall, H., and Sillard, R. Evidence for non-isostructural replacement of Zn2+ with Cd2+ in the b-domain of brain-specific metallothionein-3. (2002) FEBS Letters, 527, 76 - 80. Kangur, L and Palumaa, P. Reactivity of Metallothionein towards 5,5'-dithio-bis(2-nitrobenzoic acid). New Method for determination of binding constants for metallothionein – ligand interactions. (2001) Eur. J. Biochem., 268, 4979-4987. Vaher, M., Romero-Isart, N., Vasak, M. and Palumaa, P. Reactivity of Cd7-metallothionein with Cu(II) ions: Evidence for a cooperative formation of Cd3,Cu(I)5-metallothionein (2001) J. Inorg. Biochem. 83, 1-6. Bonetto, V., Kangur, L., Palumaa P., Mutt V., Jörnvall H., Sillard R. Large scale purification of Calbindin D9K from Porcine Intestine. (1999) Protein Expr. Purif. 17, 387 - 391. Palumaa, P. and Lehtme, R. Biochemical characterization of monomeric and dimeric cadmium-substituted metallothionein-3. In: Metallothionein IV. 1998 Birkhäuser, p. 63 – 69. Palumaa, P. and Vaher, M. Metal-induced dimerization of Cd7-metallothionein. Role of Anions. (1996) Ann. Clin. Lab. Sci. Vol. 26, No. 3, 264-268. Palumaa P., Vasak M. Formation and spectroscopic characterization of a novel monomeric cadmium- and phosphate-containing form of metallothionein. (1993) Biochemistry 32, 2874-2879. Palumaa P., Mackay E. A., Vasak M. Nonoxidative cadmium-dependent dimerization of Cd7-metallothionein from rabbit liver. (1992) Biochemistry 31, 2181-2186. Palumaa P., Vasak M. Binding of inorganic phosphate to the cadmium-induced dimeric form of metallothionein from rabbit liver. (1992) Eur. J. Biochem. 205, 1131-1135. Santa H., Laatikainen R., Lötjonen S., Palumaa P. Dynamic NMR determination of the N,N-dimethyl inversion rate and apparent Inversion free nergy in 2-hydroxy-N,N-dimethyl-2-phenylethylamine. (1992) J. Chem. Research (S), 198-199. Zorko M., Palumaa P., Kask K. Binding of some aromatic ligands to the active site of acetylcholinesterase. (1991) Periodicum Biologorum 2, 355-356. Zorko M., Langel U., Palumaa P. Simultaneous action of quaternary ammonium ligands and alkanesulfonylchlorides on electric eel acetylcholinesterase. (1990) Periodicum Biologorum 4, 385-391. Palumaa P., Kask K. Biomimetic modelling of spatial structure of acetylcholinesterase active site with cyclodextrins. (1989) Organic Reactivity 26, 122-141. Sepp A., Palumaa P., Langel U., Järv J., Zorko M. Spatial orientation of n-alkanesulfonyl chlorides in the active center of cholinesterases. (1989) Bioorg. Chem. 17, 79-85. Eller M., Järv J., Palumaa P. Influence of non-radioactive ligand on kinetics of N-methyl-[3H]scopolamine binding to muscarinic receptor. (1988) Organic reactivity 25, 372-386. Palumaa P., Järv J., Schevchenko V., Myasojedov N. Synthesis f -(N,N-dimethylamino)4,-3H-acetophenon. (1986) Radiokhim.(USSR) 22, 231-232. Palumaa P., Järv J. Alkylboronic acid acceleration of acetylcholinesterase alkylation with N,N- dimethyl-2-phenylaziridinium ion. (1985) Bioorg. Khim. (USSR) 11, 1210-1216. Palumaa P., Raidaru G., Schevchenko V., Järv J., Myasojedov N Synthesis of tritium labeled N,N- dimethyl-2-phenylaziridinium and its reaction with acetylcholinesterase. (1985) Bioorg. Khim. (USSR) 11, 1348-1352. Palumaa P., Soomets U., Järv J. Effects of reaction medium and temperature on solvolysis of N,N-dimethyl-2-phenylaziridinium ion. (1985) Organic reactivity 22, 238-257. Palumaa P., Järv J. Alkylboronic acids accelerate affinity labeling of acetylcholinesterase with N,N- dimethyl-2-phenylaziridinium ion. (1984) Biochim. Biophys. Acta 784, 35-39. Palumaa P., Raba R., Järv J. Site-specificity of butyrylcholinesterase alkylation with N,N- dimethyl-2- phenylaziridinium ion. (1984) Biochim. Biophys. Acta 791, 15-20. Palumaa P., Käämbre T., Järv J. Kinetics of the reaction of N,N-dimethyl-2-phenylaziridinium ions with muscarinic receptor and acetylcholinesterases. (1983) Bioorg. Khim. (USSR) 9, 1348-1355. Palumaa P., Mähar A,. Järv J. Kinetic analysis of butyrylcholinesterase inhibition with N,N- dimethyl-2-phenylaziridinium ion. (1982) Bioorg. Chem. 11, 394-403. |
last updated: 15.08.2005
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