| description: |
The aim of the present study is to study the immobilization of biocatalysts using the proposed integrated biosensor model, combining the kinetics of enzymatic processes, diffusin and the inertia of electrochemical sensor, but also apply this model for the calibration of biosensors, based on different biocatalysts. The present model allows the determination of biosensor kinetic and steady state parameters from the biosensor transient phase signal. These biosensor parameters can be used for the characterization of the activity of biocatalysts, used in biosensor, but also for the construction of different biosensor calibration curves. According to the aim of the analysis, it is possible to vary the determination range and accuracy by using different biosensor parameters for the calibration. Beside enzymes, it is planned to verify the model for other biocatalysts and use it for their characterization. The model can be complemented with additional members, characterising different side processes, and so eliminate their influence onto the biosensor signal. The biosensor kinetic and steady state parameters, calculated according to the presented model, allow the determination of catalytic constants of immobilised biocatalysts, where diffusional effects are eliminated and so enable the characterization of the efficiency of the immobilization process. For the calculation of these catalytic constants, the separate determinations of diffusion, partition and geometric coefficients of current biosensor configurations are not necessary, what is a great advantage of the proposed approach. The efficiency of different immobilisation methods of different enzymes and microorganisms are studied and the possibilities of stabilisation the immobilised biocatalysts are tested to apply them in biosensors for environmental monitoring, clinical analysis and biotechnology industry. |