title: Spectrofluorometric characterisation of dissolved organic matter in pore-water of lake sediments
reg no: ETF5582
project type: Estonian Science Foundation research grant
subject: 1.6-1.9. Chemistry and Molecular Biology
1.10-1.16. Bio-Geo Sciences
status: completed
institution: Institute of Ecology at Tallinn University of Educational Sciences
head of project: Aina Leeben
duration: 01.01.2003 - 31.12.2005
description: The proposed research is aimed at analysis of dissolved organic matter (DOM) in pore-water of lake sediments by using fluorescence spectra. A set of analyses (including microbiological, size exclusion chromatographic and organic matter analyses) on sediment core samples from small Estonian lakes with different trophic status will be carried out in order to:

1) investigate the relationship between fluorescence of pore-water DOM and lake historical productivity;
2) study the possibilities of using fluorescence spectra of pore-water DOM for identification of precursors of accumulated organic material.
Obtained data will be processed using principal component analysis.
Application of fluorescence technique has advantages in comparison with classical methods used in paleolimnology since it is fast and requires no sample pretreatment. Characterisation of pore-water DOM could be carried out on small volumes of samples. This fluorescence method may be useful for:
1) sorting large core sample sets to be tested by other characterisation methods or for characterisation of archived samples;
2) augment the interpretation of dissolved organic carbon sources and alterations in sediments for understanding carbon cycling in freshwater ecosystems, determination a carbon budget in aquatic systems or anthropogenous influence on lake ecosystems.

project group
no name institution position  
1.Tiiu AlliksaarInstitute of Geology TTUSenior Researcher 
2.Marina KudrjašovaTTÜ Keemia InstituutResearcher 
3.Kai KünnisInstitute of Ecology TPUResearcher 
4.Aina LeebenInstitute of Ecology at Tallinn University of Educational SciencesResearcher 
5.Viia LepaneTTULecturer