| title: | Construction of environmentally safe plant virus vectors |
|---|---|
| reg no: | ETF5909 |
| project type: | Estonian Science Foundation research grant |
| subject: |
2.7. Biotechnology, Food and Drink Technology 4.2. Crop Sciences (inc. Horticulture) |
| status: | accepted |
| institution: | TTU Faculty of Science |
| head of project: | Erkki Truve |
| duration: | 01.01.2004 - 31.12.2007 |
| description: | We propose: i) to construct a new plant virus vector based on cocksfoot mottle sobemovirus (CfMV, an efficiently propagating virus with icosahedral particles which infects monocots growing in temperate pastures); ii) to insert strong antigenic determinants from animal pathogens into the constructed vector, iii) to immunise mice with CfMV-infected plant material; iv) to compare the efficiency of vaccines expressed from differently modified CfMV-based vectors; v) to test the potential of sobemoviruses to recombine in monocot hosts and map the hotspots of recombination; vi) to mutate the possible recombination hotspots in CfMV-based vector; vii) to test the modified CfMV-based vector for potential recombinations with naturally occurring sobemoviruses; viii) to screen Estonian fields and pastures for the possible occurrence of CfMV-related sobemoviruses. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Kadri Järve | Tallinn Technical University | osakonnajuhataja | |
| 2. | Merike Meier | Tallinn Technical University | teadur | |
| 3. | Lenne Nigul | KBFI | teadur | |
| 4. | Cecilia Sarmiento | Tallinn Technical University | teadur | |
| 5. | Erkki Truve | TTU Faculty of Science | prof., director | |