| title: | Role of Gata-3 and related factors in the development of heart |
|---|---|
| reg no: | ETF5995 |
| project type: | Estonian Science Foundation research grant |
| subject: |
1.15. Zoology |
| status: | accepted |
| institution: | TU Faculty of Biology and Geography |
| head of project: | Raivo Raid |
| duration: | 01.01.2004 - 31.12.2007 |
| description: | We have shown that Gata-3-lacZ is expressed in the endocardial cushion, semilunar valves and impulse conducting system of embryonic heart of mouse and Gata-3 "knock-out" embryos have defects of outflow tract and branchial arch arteries. The homozygotes of Gata-3 mutant embryos die between 11-12 dpc and display massive internal bleeding, marked growth retradation, severe deformities of neural system (Pandolfi, et al.,1995). 1.To circumvent early embryonic lethality Gata-3 will be disrupted specifically in the heart by using cre-loxP technic. Now one can characterise the fenotype of older mutant hearts and estimate the role of Gata-3 in congenital heart diseases. Alternatively the cateholamine treatment will used to get older developmental stages. 2.To characterize changes of cellular apoptosis, proliferation, migration and differensation in the hearts with inactivated Gata-3. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Martin Kärner | TÜ,ZHI | lektor | |
| 2. | Riho Meier | o/ü Visgenyx | spetsialist | |
| 3. | Kärt Padari | TÜ,ZHI | doktorant | |
| 4. | Margus Pooga | EBK | ||
| 5. | Raivo Raid | TU Faculty of Biology and Geography | Associate Professor | |