| title: | Capillary electrophoretic monitoring in the case of limited amounts of sample |
|---|---|
| reg no: | ETF6166 |
| project type: | Estonian Science Foundation research grant |
| status: | accepted |
| institution: | Tallinn Technical University |
| head of project: | Mihkel Kaljurand |
| duration: | 01.01.2005 - 31.12.2007 |
| description: | The grant aims to demonstrate that capillary electrophoresis (CE) could form the basis for many important kinetic measurements if the monitoring of a reaction is performed by mixing the reactants within the capillary and a consecutive on-capillary separation and detection of the reactants and products takes place. The "on-capillary" assay is of particular interest since the assay and all its necessary operations completely occur within the capillary, thus reducing the volume of the assay from μL to nL. For monitoring the following sampling procedure will be implemented: the reaction and separation process is initiated many times with increasing incubation time. The timing of all events will be computer controlled. Such a multiple initiation/sampling of the reaction allows monitoring of the kinetics of reactions independently of the separation time of the analytes. We plan to introduce the concept of and demonstrate the performance of the system on an example of monitoring two oxidative processes of biomedical interest: I. The kinetics of oxidation of vitamins C and E and various plant phenols, their mixtures and plant extracts (incl. Ginkgo biloba and sea buckthorn). The importance of developing a convenient and informative assay for the determination of an antioxidative potential of various plant extracts is of importance in developing functional food products. II. The kinetics of oxidation of various intracellular peptides (e.g gluthadione). Such kinetic studies are of interest in understanding the possible mechanism of releasing oxidative stress due to insoluble protein-metal complexes and in developing drugs for treating Alzheimer’s disease. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Sille Ehala | Tallinn Technical University | PhD-student | |
| 2. | Mihkel Kaljurand | Tallinn Technical University | Professor | |
| 3. | Merike Vaher | Tallinn Technical University | researcher | |