title: Molecular biology, biochemical, morphological and nanotechnological study of luteolytic mechanisms and organ systems of transgenic mice
reg no: SF0182688s05
project type: targeted financing
subject: 3.1. Basic Medicine
status: accepted
institution: University of Tartu
head of project: Andres Arend
duration: 01.01.2005 - 31.12.2010
description: The project is formed by two sub-parts – study of corpus luteum regression or luteolysis mechanisms and cholecystokinin role in different organ systems.
Part A.Cessation of corpus luteum function and transformation into corpus albicans is still largly inexplicable. Animal model of pseudopregnancy developed in the University of Umea will be utilized and the study aims to reveal connections between localisation, kinetics and character of apoptotic cells during atresia of ovarian follicles and fuctional luteolysis using complex investigation methods (histological, immunohistochemical, electron microscopic, nanotechnological and SEM methods).
Part B. During the last decades progressively more attention has been paid for develeopment and investigation of transgenic animals. Mice models because of their compact nature and short life cycle give broad perspectives for modelling pathological conditions, which could take years in humans. The aim of the study is to investigate morphological changes in organs (brain, cerebellum, testis, stomach, kidney) of mice with cholecystokinin overexpression (NSE-CCK), cholecystokinin CCK2 receptor insufficiency and wolframin gene deficiency using histologyical, immunohistological, electron microscopical (EM, EM+immunohistology, SEM), in situ hybridisation and nanotechnological methods in normal and pathological conditions.

project group
no name institution position  
1.Vladimir AndrianovTartu University, Faculty of MedicineAssociated professor 
2.Andres ArendTartu University, Faculty of MedicineProfessor, head of the Department of Anatomy 
3.Marina AunapuuTartu University, Faculty of MedicineAssociated Professor (0,5); senior research fellow (0,5) 
4.Sulev IngerpuuTartu University, Faculty of MedicineAssistant Professor 
5.Tõnu JärveotsTartu University, Faculty of Medicinelecturer 
6.Mart LintsiTartu University, Faculty of Medicinespecialist, PHD-student 
7.Raivo MassoTartu University, Faculty of MedicineAssociate professor 
8.Agu RaudheidingTartu University, Faculty of MedicineLaboratory specialist 
9.Peeter RoosaarTartu University, Faculty of Medicinedocent emeritus 
10.Kristjan SaalTartu University, Faculty of Medicinescientist 
11.Helle-Evi SimovartTartu University, Faculty of Medicineresearcher 
12.Toivo SuurojaTartu University, Faculty of MedicineProfessor