| title: | Studies of RNA polymerase II dependent elongation of transcription |
|---|---|
| reg no: | ETF6604 |
| project type: | Estonian Science Foundation research grant |
| subject: |
1.8. Molecular Biology 1.9. Genetics, Physiology and Microbiology |
| status: | accepted |
| institution: | TU Faculty of Biology and Geography |
| head of project: | Arnold Kristjuhan |
| duration: | 01.01.2006 - 31.12.2009 |
| description: | For efficient transcription of eukaryotic genes, RNA polymerase has to contend with nucleosomes and higher structures of chromatin to gain access to DNA. Although chromatin remodeling on promoter regions of genes has been intensively studied during past decade, the mechanisms, how elongating RNA polymerase can cope with chromatin covering long regions of coding sequences, is still poorly understood. The aim of proposed research is to determine the changes in the structure of chromatin (modifications of histones, removal of nucleosomes, etc) in response to transcriptional elongation of RNA polymerase II, also to determine the cellular factors needed for modification of chromatin during transcriptional elongation of RNA polymerase II. We also plan to investigate what are the mechanisms and cellular factors needed for restoration of chromatin structure upon shut-down of transcription. Additionaly, we will develop an in vivo genetic screen for novel factors involved in transcriptional elongation on chromosomal DNA. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Jaak Jänes | |||
| 2. | Arnold Kristjuhan | Tartu Ülikool | ||
| 3. | Signe Värv | |||