| title: | Pathogenesis of heterotopic ossification in humans and rats |
|---|---|
| reg no: | ETF5445 |
| project type: | Estonian Science Foundation research grant |
| subject: |
3. Medical Sciences |
| status: | accepted |
| institution: | TU Faculty of Medicine |
| head of project: | Andres Arend |
| duration: | 01.01.2003 - 31.12.2006 |
| description: | The main aim of the project is to study the pathogenesis of heterotopic ossification (HO), which is a frequent complication of endoprosthetic operations.The study will be performed both on the clinical material and on rats. The exact tasks of the project are: 1. To determine the expression of genes for agents contributing in osteoneogenesis BMP-2, TGF-b1, TGF-b2, TGF-b3 and the levels of prostaglandins PGE2 ja PGF2a in human ossificates formed after hip replacement. 2. To compare the gene expression and levels of these agents in different zones achived by micropreparation of ossificate at different time-points. 3. To study above mentioned parameters in preoperative control samples and to analyze the association between their values and later HO formation. 4. To establish an HO experimental model, which is well standardized regarding the source of stem cells and resambles HO formation following endoprostehetic replacement in humans. 5. To study the temporal dynamics of expression of BMP-2, TGF-b1, TGF-b2, TGF-b3, COX-1 and COX-2 genes and the content of PGs and IL-6 in different zones of experimental ossificate. 6. To elaborate the RNA extraction method based on different histological zones of the ossificate, which allows to study ossificate cells in vivo both in human and in rat. Investigation of the pathogenetic mechanisms of HO on samples harvested from humans helps to understand better the process and to optimize the treatment schedule. New knowledge about the peculiarities of growth factor production in HO may give new ideas for elaboration of novel treatment methods. Animal model gives us possibility to study experimentally the role of bone marrow stroma in occurrence of HO and may also have direct influence on the operative methods choosen in clinical practice. Application and validation the RNA-extraction method in one model opens possibilities to alpply it also for in vivo investigation of other connective tissue and bone diseases and pathologic conditions. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Andres Arend | TU Faculty of Medicine | docent | |
| 2. | Aare Märtson | Tartu Univ Clinic | head of dept | |
| 3. | Leho Rips | Univ of Tartu | resident | |
| 4. | Peeter Roosaar | Univ of Tartu | docent | |
| 5. | Alar Toom | Univ of Tartu | doctoral student | |