| title: | The role of rRNA mdofications in the ribosome |
|---|---|
| reg no: | ETF5822 |
| project type: | Estonian Science Foundation research grant |
| subject: |
1.8. Molecular Biology |
| status: | accepted |
| institution: | TU Faculty of Biology and Geography |
| head of project: | Jaanus Remme |
| duration: | 01.01.2004 - 31.12.2007 |
| description: | Resolution of ribosome stracture at high-resolution by X-ray crystallography and cryo-electron microscopy in recent years has made it it possible to study structure-function relation in ribosomes at atomic resolution. Some questions on the ribosome functioning asked already long time ago can be solved. One of the old questions in ribosome research is the function of modified nucleosides find in all high-molcular rRNA species studied. In this project we propse to analyze the functioning of modified nucleosides in rRNA both in ribosome biogenesis and in ribosome functioning. The main approach is to use mutant strains of E. coli lacking one or more rRNA modification enzyme or containing mutations in the rRNA genes. Ribosome assembly defects are manifested by the accumulation of subunit assembly precursor particles, which can be visualized by sucrose gradient centrifugation. It is evident from published results and from our prelimiray data that several modification enzymes play an active role in ribosome assembly. We will also identify when the modifications are made during ribosome assembly. Ribosome subunit assembly proceeds in several steps. In addition, we will analyze translational accuracy in modification deficeint strains. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Ülar Allas | University of Tartu | doktorant | |
| 2. | Aivar Liiv | EBK | vanemteadur | |
| 3. | Ülo Maiväli | University of Tartu | ||
| 4. | Lauri Peil | University of Tartu | doktorant | |
| 5. | Jaanus Remme | TU Faculty of Biology and Geography | professor | |
| 6. | Kai Virumäe | vanemlaborant | ||